Description of the metallo-ß-lactamase GIM-1 in Acinetobacter pittii.
J Antimicrob Chemother
; 69(1): 81-4, 2014 Jan.
Article
em En
| MEDLINE
| ID: mdl-23956376
ABSTRACT
OBJECTIVES:
To characterize the mechanisms involved in the reduced carbapenem susceptibility of five Acinetobacter pittii strains isolated from different regions of Germany.METHODS:
The strains were analysed by susceptibility testing, phenotypic tests for metallo-ß-lactamase production, sequencing of the integron structure and strain typing by PFGE, as well as multilocus sequence typing (MLST) and plasmid analysis by S1 restriction and hybridization.RESULTS:
Despite GIM-1 production, the MICs of imipenem were only 4 mg/L for four strains and some methods of phenotypic MBL detection failed. According to PFGE and MLST, the strains belonged to four different clones, but blaGIM-1 was present in identical integron structures in all strains and carried on plasmids of â¼60 kb.CONCLUSIONS:
For the first time, GIM-1 has been demonstrated in A. pittii. This resistance mechanism has previously been reported only in Enterobacteriaceae and Pseudomonas aeruginosa. As GIM-1 was found in strains with diverse clonal backgrounds, but encoded on plasmids of a similar size, further spread among Acinetobacter spp. seems possible. The detection of GIM-1 production might be challenging in some strains due to the low MICs of carbapenems.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Acinetobacter
/
Beta-Lactamases
/
Carbapenêmicos
/
Resistência beta-Lactâmica
Limite:
Aged
/
Aged80
/
Humans
/
Male
/
Middle aged
País/Região como assunto:
Europa
Idioma:
En
Revista:
J Antimicrob Chemother
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Alemanha