A novel model for orthotopic liver transplantation in rats using hepatic rearterialization and biliary extradrainage system.
J Surg Res
; 187(2): 690-8, 2014 Apr.
Article
em En
| MEDLINE
| ID: mdl-24345526
ABSTRACT
BACKGROUND:
Although the rat orthotopic liver transplantation (OLT) model has existed for many years, only a few models can be applied for dynamic bile collection. The aim of this study was to introduce a dependent rat OLT model with hepatic rearterialization and an expediently dynamic bile collection system.METHODS:
Forty-five male Sprague-Dawley rats were divided into the following three groups (n = 15 each) group A, OLT without hepatic rearterialization; group B, OLT with hepatic rearterialization; group C, OLT with hepatic rearterialization and a biliary extradrainage system. In groups B and C, a modified sleeve anastomosis between the donor common hepatic artery and the recipient proper hepatic artery was performed to restore the hepatic artery blood flow. In group C, after hepatic rearterialization, biliary extradrainage and jejunum stoma were performed to reestablish the bile flow, and a waistcoat-like external fixator was introduced to protect this system.RESULTS:
The surgical success rates in groups A, B, and C were 100% (15/15), 93% (14/15), and 93% (14/15), respectively. In groups B and C, the hepatic artery patency rates were 93% and 86% on postoperative day 3 and postoperative day 21, respectively. Also, the liver function and bile duct integrity were preserved better than that in group A. In group C, the biliary extradrainage system was well preserved and bile collection was easily performed.CONCLUSIONS:
The rat OLT model with hepatic rearterialization and a convenient biliary extradrainage system was satisfactory in maintaining the survival rate, hepatic artery patency rate, and recovery of graft function, so it can be applied in various studies after transplantation.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Procedimentos Cirúrgicos Vasculares
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Procedimentos Cirúrgicos do Sistema Biliar
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Transplante de Fígado
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Ratos Sprague-Dawley
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Modelos Animais
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Artéria Hepática
Limite:
Animals
Idioma:
En
Revista:
J Surg Res
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
China