Growth inhibition and differences in protein profiles in azadirachtin-treated Drosophila melanogaster larvae.
Electrophoresis
; 35(8): 1122-9, 2014 Apr.
Article
em En
| MEDLINE
| ID: mdl-24458307
ABSTRACT
Azadirachtin A is a very effective biopesticide widely used in insect pest control. It has strong antifeeding and growth inhibitory activity against most insects, however, its mode of action is still unclear. Proteomic experiments using 2DE indicate significant effects of Azadirachtin A on the amount of proteins related to growth inhibition in Drosophila melanogaster larvae. Twenty-one spots with different intensity in azadirachtin-treated larvae were identified. These proteins are involved in cytoskeletal organization, transcription and translation, hormonal regulation, and energy metabolism. Protein network analysis reveals heat shock protein 23 to be a potential target of azadirachtin. These results provide new insights into understanding the mechanism of growth inhibition in insects in response to azadirachtin.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Eletroforese em Gel Bidimensional
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Proteínas de Drosophila
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Limoninas
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Drosophila melanogaster
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Inseticidas
Limite:
Animals
Idioma:
En
Revista:
Electrophoresis
Ano de publicação:
2014
Tipo de documento:
Article