Measurement of corneal elasticity with an acoustic radiation force elasticity microscope.
Ultrasound Med Biol
; 40(7): 1671-9, 2014 Jul.
Article
em En
| MEDLINE
| ID: mdl-24726798
ABSTRACT
To investigate the role of collagen structure in corneal biomechanics, measurement of localized corneal elasticity with minimal destruction to the tissue is necessary. We adopted the recently developed acoustic radiation force elastic microscopy (ARFEM) technique to measure localize biomechanical properties of the human cornea. In ARFEM, a low-frequency, high-intensity acoustic force is used to displace a femtosecond laser-generated microbubble, while high-frequency, low-intensity ultrasound is used to monitor the position of the microbubble within the cornea. Two ex vivo human corneas from a single donor were dehydrated to physiologic thickness, embedded in gelatin and then evaluated using the ARFEM technique. In the direction perpendicular to the corneal surface, ARFEM measurements provided elasticity values of E = 1.39 ± 0.28 kPa for the central anterior cornea and E = 0.71 ± 0.21 kPa for the central posterior cornea in pilot studies. The increased value of corneal elasticity in the anterior cornea correlates with the higher density of interweaving lamellae in this region.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Interpretação de Imagem Assistida por Computador
/
Córnea
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Microscopia Acústica
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Técnicas de Diagnóstico Oftalmológico
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Técnicas de Imagem por Elasticidade
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Testes de Dureza
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Ultrasound Med Biol
Ano de publicação:
2014
Tipo de documento:
Article