[Effects of airborne fine particulate matters on pulmonary inflammation injury and Th17/Treg balance of sub-chronic exposure mice].
Wei Sheng Yan Jiu
; 43(3): 387-92, 2014 May.
Article
em Zh
| MEDLINE
| ID: mdl-24964615
ABSTRACT
OBJECTIVE:
To explore effects of fine particulate matters (PM 2.5) onpulmonary inflammation, and the changes of Th17/Treg balance as well as related cytokines.METHOD:
Thirty-two C57BL/6 male mice were randomly divided into 4 groups including 1 saline control group and 3 PM2.5 exposure groups (1.5, 7.5 and 15 mg/kg BW, respectively). Each group received intratracheal instillation twice per week for 3 consecutive months. 24 hours after the last exposure, anaesthetize the mice with chloral hydrate, bronchoalveolar lavage fluid (BALF) was collected for inflammatory cells and cytokines analysis. The Th17- and Treg-related cytokines in BALF was measured by enzyme linked immunosorbent assay (ELISA). The level of the specific transcription factors of Th17 and Treg in lung tissue was determined by real-time PCR. Unlavaged left lung were fixed with 4% paraformaldehyde for histopathological detection.RESULTS:
Th17-related cytokine IL-17 increased, but Treg-related cytokine IL-10 decreased significantly in BALF at 7.5 and 15 mg/kg BW PM2.5 exposure groups compared with control group (P < 0.05). Consistently, the relative mRNA expression of ROR-gammat (specific transcription factors of Th17) increased in a dose-response way, the relative mRNA expression of Foxp3 + (specific transcription factors of Treg) decreased in a dose-response way.CONCLUSION:
Sub-chronic PM2.5 exposure caused persistent inflammation, immune injury and disordered the Th17/Treg imbalance as well as related cytokines.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poluentes Atmosféricos
/
Material Particulado
Limite:
Animals
Idioma:
Zh
Revista:
Wei Sheng Yan Jiu
Assunto da revista:
SAUDE PUBLICA
Ano de publicação:
2014
Tipo de documento:
Article