Crystallization of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34.

ABSTRACT

The phage-proximal part of the long tail fibres of bacteriophage T4 consists of a trimer of the 1289 amino-acid gene product 34 (gp34). Different carboxy-terminal parts of gp34 have been produced and crystallized. Crystals of gp34(726-1289) diffracting X-rays to 2.9 Šresolution, crystals of gp34(781-1289) diffracting to 1.9 Šresolution and crystals of gp34(894-1289) diffracting to 3.0 and 2.0 Šresolution and belonging to different crystal forms were obtained. Native data were collected for gp34(726-1289) and gp34(894-1289), while single-wavelength anomalous diffraction data were collected for selenomethionine-containing gp34(781-1289) and gp34(894-1289). For the latter, high-quality anomalous signal was obtained.