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Phenotype alterations in regulatory T-cell subsets in primary HIV infection and identification of Tr1-like cells as the main interleukin 10-producing CD4+ T cells.
Chevalier, Mathieu F; Didier, Céline; Petitjean, Gaël; Karmochkine, Marina; Girard, Pierre-Marie; Barré-Sinoussi, Françoise; Scott-Algara, Daniel; Weiss, Laurence.
Afiliação
  • Chevalier MF; Régulation des infections rétrovirales, Institut Pasteur Université Paris Diderot.
  • Didier C; Régulation des infections rétrovirales, Institut Pasteur.
  • Petitjean G; Régulation des infections rétrovirales, Institut Pasteur.
  • Karmochkine M; Hôpital Européen Georges Pompidou.
  • Girard PM; Hôpital Saint Antoine, Assistance Publique-Hôpitaux de Paris, France.
  • Barré-Sinoussi F; Régulation des infections rétrovirales, Institut Pasteur.
  • Scott-Algara D; Régulation des infections rétrovirales, Institut Pasteur.
  • Weiss L; Régulation des infections rétrovirales, Institut Pasteur Université Paris Descartes, Sorbonne Paris Cité Hôpital Européen Georges Pompidou.
J Infect Dis ; 211(5): 769-79, 2015 Mar 01.
Article em En | MEDLINE | ID: mdl-25281758
BACKGROUND: Conventional regulatory T cells (Tregs) can suppress human immunodeficiency virus type 1 (HIV-1)-specific immune responses but cannot control immune activation in primary HIV infection. Here, we characterized Treg subsets, using recently defined phenotypic delineation, and analyzed the relative contribution of cell subsets to the production of immunosuppressive cytokines in primary HIV infection. METHODS: In a longitudinal prospective study, ex vivo phenotyping of fresh peripheral blood mononuclear cells from patients with primary HIV infection was performed at baseline and month 6 of follow-up to characterize Treg subsets, immune activation, and cytokine production in isolated CD4(+) T cells. RESULTS: The frequency of CD4(+)CD25(+)CD127(low) Tregs and the distribution between the naive, memory, and activated/memory Treg subsets was similar in patients and healthy donors. However, Tregs from patients with primary HIV infection showed peculiar phenotypic profiles, such as elevated FoxP3, ICOS, and CTLA-4 expression, with CTLA-4 expression strikingly increased in all Treg subsets both at baseline and month 6 of follow-up. The great majority of interleukin 10 (IL-10)-producing CD4(+) T cells were FoxP3(neg) (ie, Tr1-like cells). In contrast to conventional Tregs, Tr1-like cells were inversely correlated with immune activation and not associated with lower effector T-cell responses. CONCLUSION: FoxP3(neg) Tr1-like cells-major contributors to IL-10 production-may have a beneficial role by controlling immune activation in early HIV infection.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por HIV / Imunofenotipagem / Subpopulações de Linfócitos T / Interleucina-10 / Linfócitos T Reguladores Tipo de estudo: Diagnostic_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: J Infect Dis Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por HIV / Imunofenotipagem / Subpopulações de Linfócitos T / Interleucina-10 / Linfócitos T Reguladores Tipo de estudo: Diagnostic_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: J Infect Dis Ano de publicação: 2015 Tipo de documento: Article