[AFLP analysis on genetic diversity of Angelica sinensis].
Zhong Yao Cai
; 37(4): 572-5, 2014 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-25345126
ABSTRACT
OBJECTIVE:
To explore the genetic diversity and relationship of different germplasm of Angelica sinensis.METHODS:
Amplified fragment length polymorphism (AFLP) markers were developed to analyze genetic polymorphism in 12 populations 117 samples of Angelica sinensis. The amplified fragments were used for cluster analysis among the different germplasm of Angelica sinensis and to construct the genetic phylogenetic tree with NTSYSpc 2. 11 software.RESULTS:
Eight primer combinations selected from 64 primer combinations were used for amplification and a total of 815 fragments were obtained. Analysis identified 812 polymorphic fragments, accounting for 99.63% of the total detected fragments. Different phenotypes and germplasm of Angelica sinensis could be divided by genetic phylogenetic tree analysis.CONCLUSION:
AFLP molecular markers can indicate the significant polymorphism and genetic diversity among germplasm resources of Angelica sinensis. The cultivated purple-stemmed and green-stemmed phenotypes of Angelica sinensis may have different genotypes. The results can provide theoretical evidence for reasonable utilization and breeding new cultivar in molecular level.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Variação Genética
/
Angelica sinensis
/
Análise do Polimorfismo de Comprimento de Fragmentos Amplificados
Idioma:
Zh
Revista:
Zhong Yao Cai
Assunto da revista:
TERAPIAS COMPLEMENTARES
Ano de publicação:
2014
Tipo de documento:
Article