A multiplex RT-PCR for simultaneous detection and identification of five viruses and two viroids infecting chrysanthemum.
Arch Virol
; 160(5): 1145-52, 2015 May.
Article
em En
| MEDLINE
| ID: mdl-25698104
ABSTRACT
Pathogens causing significant economic losses in chrysanthemum include tomato aspermy virus (TAV), chrysanthemum virus B (CVB), cucumber mosaic virus (CMV), tobacco mosaic virus (TMV), potato virus Y (PVY), chrysanthemum stunt viroid (CSVd) and chrysanthemum chlorotic mottle viroid (CChMVd). A multiplex reverse transcription polymerase chain reaction (RT-PCR) method, using specific primer sets for each virus or viroid, was developed for simultaneous detection and differentiation of TAV, CVB, CMV, TMV, PVY, CChMVd, and CSVd. The RT-PCR method was validated by testing chrysanthemum samples collected from different regions of China. In this study, CVB, TAV, TMV, PVY, CSVd, CMV, and CChMVd were detected, respectively, in 24.7 %, 17.5 %, 4.4 %, 4.4 %, 2.9 %, 2.5 %, and 1.5 % of the samples tested. These results indicate that CVB and TAV (24.7 % and 17.5 %) are common, whereas CMV, TMV, CChMVd, CSVd, and PVY (all below 5 %) are less frequently encountered. This new multiplex RT-PCR method has potential to be used routinely in large-scale virus and viroid surveys.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus de RNA
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Viroides
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Virologia
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Técnicas de Diagnóstico Molecular
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Chrysanthemum
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Reação em Cadeia da Polimerase Multiplex
Tipo de estudo:
Diagnostic_studies
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Prevalence_studies
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Risk_factors_studies
País/Região como assunto:
Asia
Idioma:
En
Revista:
Arch Virol
Ano de publicação:
2015
Tipo de documento:
Article