Tracking a protein following dissociation from a protein-protein complex using a split SNAP-tag system.
Anal Biochem
; 477: 53-5, 2015 May 15.
Article
em En
| MEDLINE
| ID: mdl-25724549
ABSTRACT
Protein-protein interactions (PPIs) are important for various biological processes in living cells. Several methods have been developed for the visualization of PPIs in vivo; however, these methods are unsuitable for visualization of post-PPI events such as dissociation and translocation. In this study, we applied a split SNAP-tag system for the visualization of post-PPI events. This method enabled tracking of the protein following dissociation from the protein-protein complex. Thus, the split SNAP-tag system should prove to be a useful tool for visualization of post-PPI events.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
O(6)-Metilguanina-DNA Metiltransferase
/
Mapeamento de Interação de Proteínas
Limite:
Humans
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2015
Tipo de documento:
Article