Tracking a protein following dissociation from a protein-protein complex using a split SNAP-tag system.

ABSTRACT

Protein-protein interactions (PPIs) are important for various biological processes in living cells. Several methods have been developed for the visualization of PPIs in vivo; however, these methods are unsuitable for visualization of post-PPI events such as dissociation and translocation. In this study, we applied a split SNAP-tag system for the visualization of post-PPI events. This method enabled tracking of the protein following dissociation from the protein-protein complex. Thus, the split SNAP-tag system should prove to be a useful tool for visualization of post-PPI events.