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Design and Synthesis of an Artificial Pulmonary Pleura for High Throughput Studies in Acellular Human Lungs.
Wagner, Darcy E; Fenn, Spencer L; Bonenfant, Nicholas R; Marks, Elliot R; Borg, Zachary; Saunders, Patrick; Floreani, Rachael A; Weiss, Daniel J.
Afiliação
  • Wagner DE; Department of Medicine, University of Vermont, 226 Health Sciences Research Facility Center, Burlington, VT 05405 USA.
  • Fenn SL; College of Engineering and Mathematical Sciences, University of Vermont, 109 Votey Hall, Burlington, VT 05405 USA.
  • Bonenfant NR; Department of Medicine, University of Vermont, 226 Health Sciences Research Facility Center, Burlington, VT 05405 USA.
  • Marks ER; Department of Medicine, University of Vermont, 226 Health Sciences Research Facility Center, Burlington, VT 05405 USA.
  • Borg Z; Department of Medicine, University of Vermont, 226 Health Sciences Research Facility Center, Burlington, VT 05405 USA.
  • Saunders P; Department of Medicine, University of Vermont, 226 Health Sciences Research Facility Center, Burlington, VT 05405 USA.
  • Floreani RA; College of Engineering and Mathematical Sciences, University of Vermont, 109 Votey Hall, Burlington, VT 05405 USA.
  • Weiss DJ; Department of Medicine, University of Vermont, 226 Health Sciences Research Facility Center, Burlington, VT 05405 USA.
Cell Mol Bioeng ; 7(2): 184-195, 2014 Jun.
Article em En | MEDLINE | ID: mdl-25750684
Whole organ decellularization of complex organs, such as lungs, presents a unique opportunity for use of acellular scaffolds for ex vivo tissue engineering or for studying cell-extracellular matrix interactions ex vivo. A growing body of literature investigating decellularizing and recellularizing rodent lungs has provided important proof of concept models and rodent lungs are readily available for high throughput studies. In contrast, comparable progress in large animal and human lungs has been impeded owing to more limited availability and difficulties in handling larger tissue. While the use of smaller segments of acellular large animal or human lungs would maximize usage from a single lung, excision of small acellular segments compromises the integrity of the pleural layer, leaving the terminal ends of blood vessels and airways exposed. We have developed a novel pleural coating using non-toxic ionically crosslinked alginate or photocrosslinked methacrylated alginate which can be applied to excised acellular lung segments, permits inflation of small segments, and significantly enhances retention of cells inoculated through cannulated airways or blood vessels. Further, photocrosslinking methacrylated alginate, using eosin Y and triethanolamine at 530 nm wavelength, results in a mechanically stable pleural coating that permits effective cyclic 3-dimensional stretch, i.e., mechanical ventilation, of individual segments.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Cell Mol Bioeng Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Cell Mol Bioeng Ano de publicação: 2014 Tipo de documento: Article