Routine HLA-DP typing by RFLP analysis.

ABSTRACT

HLA-DP typing using the Primed Lymphocyte Test (PLT) is a long and cumbersome technique requiring DP sensitized clones and bulk reagents. We describe here the use of restriction fragment length polymorphism (RFLP) analysis. This method is quicker and was found to be reliable after comparative analysis of the results between PLT and RFLP in 15 local families and 72 core cell lines from the Tenth International Histocompatibility Workshop.