Simple and rapid determination of unsaturated fatty acids in 1 µl of rat plasma by LC-MS/MS.
Bioanalysis
; 7(9): 1081-91, 2015.
Article
em En
| MEDLINE
| ID: mdl-26039806
ABSTRACT
BACKGROUND:
Deficiency or imbalance of unsaturated fatty acids will promote the pathogenesis of many diseases. In order to monitor the exposure of unsaturated fatty acids, the method based on LC-MS/MS was developed.RESULTS:
Standard calibration curves for α-linolenic acid, linoleic acid, palmitoleic acid and oleic acid were linear (r ≥0.99). The intra-and interbatch accuracy (RE%) ranged from -4.5 to 8.6%, while the intra- and interbatch precisions (RSD%) were ≤8.7%. The extraction recovery varied from 85.4 to 99.6%, and no obvious matrix effect was observed.CONCLUSION:
The method offers a simple approach for measuring 4 unsaturated fatty acids in 1 µl rat plasma within 3.95 min.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Análise Química do Sangue
/
Cromatografia Líquida
/
Espectrometria de Massas em Tandem
/
Ácidos Graxos Insaturados
Limite:
Animals
Idioma:
En
Revista:
Bioanalysis
Ano de publicação:
2015
Tipo de documento:
Article