Practical and low cost whole-organism motility assay: A step-by-step protocol.
Mol Cell Probes
; 30(1): 13-7, 2016 Feb.
Article
em En
| MEDLINE
| ID: mdl-26365227
ABSTRACT
Here, we provide a step-by-step protocol for a practical and low cost whole-organism assay for the screening of chemical compounds for activity against parasitic worms. This assay has considerable advantages over conventional methods, mainly in relation to ease of use, throughput, time and cost. It is readily suited to the screening of hundreds to thousands of compounds for subsequent hit-to-lead optimisation, and should be applicable to many different parasites and other organisms commensurate with the size of wells in the microtiter plates used for phenotypic screening.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Estrongilídios
/
Ensaios de Triagem em Larga Escala
/
Locomoção
/
Antinematódeos
Tipo de estudo:
Guideline
/
Health_economic_evaluation
Limite:
Animals
Idioma:
En
Revista:
Mol Cell Probes
Assunto da revista:
BIOLOGIA MOLECULAR
/
BIOTECNOLOGIA
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Austrália