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Practical and low cost whole-organism motility assay: A step-by-step protocol.
Preston, Sarah; Jabbar, Abdul; Nowell, Cameron; Joachim, Anja; Ruttkowski, Baerbel; Cardno, Tony; Hofmann, Andreas; Gasser, Robin B.
Afiliação
  • Preston S; Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia.
  • Jabbar A; Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia.
  • Nowell C; Monash University Institute of Pharmaceutical Sciences (MIPS), Monash University, Parkville, Victoria 3052, Australia.
  • Joachim A; Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinärstrasse 1, A-1210 Vienna, Austria.
  • Ruttkowski B; Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinärstrasse 1, A-1210 Vienna, Austria.
  • Cardno T; Monash University Institute of Pharmaceutical Sciences (MIPS), Monash University, Parkville, Victoria 3052, Australia.
  • Hofmann A; Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia; Structural Chemistry Program, Eskitis Institute, Griffith University, Brisbane, Queensland 4111, Australia.
  • Gasser RB; Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia. Electronic address: robinbg@unimelb.edu.au.
Mol Cell Probes ; 30(1): 13-7, 2016 Feb.
Article em En | MEDLINE | ID: mdl-26365227
ABSTRACT
Here, we provide a step-by-step protocol for a practical and low cost whole-organism assay for the screening of chemical compounds for activity against parasitic worms. This assay has considerable advantages over conventional methods, mainly in relation to ease of use, throughput, time and cost. It is readily suited to the screening of hundreds to thousands of compounds for subsequent hit-to-lead optimisation, and should be applicable to many different parasites and other organisms commensurate with the size of wells in the microtiter plates used for phenotypic screening.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Estrongilídios / Ensaios de Triagem em Larga Escala / Locomoção / Antinematódeos Tipo de estudo: Guideline / Health_economic_evaluation Limite: Animals Idioma: En Revista: Mol Cell Probes Assunto da revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Austrália
Texto completo
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PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Estrongilídios / Ensaios de Triagem em Larga Escala / Locomoção / Antinematódeos Tipo de estudo: Guideline / Health_economic_evaluation Limite: Animals Idioma: En Revista: Mol Cell Probes Assunto da revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Austrália