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A simple method for the formalin fixation of lungs in toxicological pathology studies.
Qu, Wen-Sheng; Yin, Ji-Ye; Wang, He-Mei; Dong, Yan-Sheng; Ding, Ri-Gao.
Afiliação
  • Qu WS; State Key Laboratory of Toxicology and Medical Countermeasures, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China.
  • Yin JY; State Key Laboratory of Toxicology and Medical Countermeasures, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China.
  • Wang HM; State Key Laboratory of Toxicology and Medical Countermeasures, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: whemei@ailyun.com.
  • Dong YS; State Key Laboratory of Toxicology and Medical Countermeasures, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China.
  • Ding RG; State Key Laboratory of Toxicology and Medical Countermeasures, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: dingrigao@bmi.ac.cn.
Exp Toxicol Pathol ; 67(10): 533-8, 2015 Oct.
Article em En | MEDLINE | ID: mdl-26388042
ABSTRACT
Optimized lung preparation for detailed structural evaluation is required to improve consistency in preclinical safety evaluation, differences of opinion exist among regulatory agency personnel regarding the optimal methods for routine formalin fixation of lungs from rodent toxicology studies. The simple tracheal ligation fixation method emphasizes tracheal ligation before opening the thorax instead of attempting to re-inflate after lung collapse when opening the thorax. Photomicrographs of this method demonstrated an unprecedented ability to maintain the natural lung architecture, in contrast to the unavoidable changes in the alveolar environment by the intratracheal instillation and vascular perfusion methods. In addition, a comparison of fixation methods on lung morphology in a rodent model of LPS-induced acute lung injury demonstrated that the tracheal ligation fixation method may provide a standard approach for morphometry. Additionally, a TUNEL assay was used to determine the degree of autolysis, which revealed that the autolysis was insignificant in the central areas of each lobe of the lung compared to the lung periphery by tracheal ligation fixation. In conclusion, our novel modified method, which avoids the disadvantages of generating artifacts, fulfills the requirement of preserving the clear, natural morphology of the lung making it suitable and worthy of recommendation for toxicological studies in a good laboratory practice (GLP) lab.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Patologia / Toxicologia / Fixação de Tecidos / Pulmão Tipo de estudo: Guideline Limite: Animals Idioma: En Revista: Exp Toxicol Pathol Assunto da revista: PATOLOGIA / TOXICOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Patologia / Toxicologia / Fixação de Tecidos / Pulmão Tipo de estudo: Guideline Limite: Animals Idioma: En Revista: Exp Toxicol Pathol Assunto da revista: PATOLOGIA / TOXICOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China