Lifetime estimation of moving subcellular objects in frequency-domain fluorescence lifetime imaging microscopy.
J Opt Soc Am A Opt Image Sci Vis
; 32(10): 1821-35, 2015 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-26479936
ABSTRACT
Fluorescence lifetime is usually defined as the average nanosecond-scale delay between excitation and emission of fluorescence. It has been established that lifetime measurements yield numerous indications on cellular processes such as interprotein and intraprotein mechanisms through fluorescent tagging and Förster resonance energy transfer. In this area, frequency-domain fluorescence lifetime imaging microscopy is particularly appropriate to probe a sample noninvasively and quantify these interactions in living cells. The aim is then to measure the fluorescence lifetime in the sample at each location in space from fluorescence variations observed in a temporal sequence of images obtained by phase modulation of the detection signal. This leads to a sensitivity of lifetime determination to other sources of fluorescence variations such as intracellular motion. In this paper, we propose a robust statistical method for lifetime estimation for both background and small moving structures with a focus on intracellular vesicle trafficking.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
J Opt Soc Am A Opt Image Sci Vis
Assunto da revista:
OFTALMOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article