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High-resolution mass spectrometry of small molecules bound to membrane proteins.
Gault, Joseph; Donlan, Joseph A C; Liko, Idlir; Hopper, Jonathan T S; Gupta, Kallol; Housden, Nicholas G; Struwe, Weston B; Marty, Michael T; Mize, Todd; Bechara, Cherine; Zhu, Ya; Wu, Beili; Kleanthous, Colin; Belov, Mikhail; Damoc, Eugen; Makarov, Alexander; Robinson, Carol V.
Afiliação
  • Gault J; Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, UK.
  • Donlan JA; Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, UK.
  • Liko I; Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, UK.
  • Hopper JT; Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, UK.
  • Gupta K; Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, UK.
  • Housden NG; Department of Biochemistry, University of Oxford, Oxford, UK.
  • Struwe WB; Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, UK.
  • Marty MT; Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, UK.
  • Mize T; Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, UK.
  • Bechara C; Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, UK.
  • Zhu Y; CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Pudong, China.
  • Wu B; CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Pudong, China.
  • Kleanthous C; Department of Biochemistry, University of Oxford, Oxford, UK.
  • Belov M; Thermo Fisher Scientific, Bremen, Germany.
  • Damoc E; Thermo Fisher Scientific, Bremen, Germany.
  • Makarov A; Thermo Fisher Scientific, Bremen, Germany.
  • Robinson CV; Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, UK.
Nat Methods ; 13(4): 333-6, 2016 Apr.
Article em En | MEDLINE | ID: mdl-26901650
Small molecules are known to stabilize membrane proteins and to modulate their function and oligomeric state, but such interactions are often hard to precisely define. Here we develop and apply a high-resolution, Orbitrap mass spectrometry-based method for analyzing intact membrane protein-ligand complexes. Using this platform, we resolve the complexity of multiple binding events, quantify small molecule binding and reveal selectivity for endogenous lipids that differ only in acyl chain length.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fragmentos de Peptídeos / Espectrometria de Massas / Bibliotecas de Moléculas Pequenas / Lipídeos / Proteínas de Membrana Limite: Humans Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2016 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fragmentos de Peptídeos / Espectrometria de Massas / Bibliotecas de Moléculas Pequenas / Lipídeos / Proteínas de Membrana Limite: Humans Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2016 Tipo de documento: Article