High-Throughput Measurement of Binding Kinetics by mRNA Display and Next-Generation Sequencing.

Jalali-Yazdi, Farzad; Lai, Lan Huong; Takahashi, Terry T; Roberts, Richard W

Jalali-Yazdi, Farzad; Lai, Lan Huong; Takahashi, Terry T; Roberts, Richard W.

Afiliação

Jalali-Yazdi F; Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, CA, 90089, USA.
Lai LH; Department of Chemistry, University of Southern California, Los Angeles, CA, 90089, USA.
Takahashi TT; Department of Chemistry, University of Southern California, Los Angeles, CA, 90089, USA.
Roberts RW; Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, CA, 90089, USA. richrob@usc.edu.

Angew Chem Int Ed Engl ; 55(12): 4007-10, 2016 Mar 14.

Article em En | MEDLINE | ID: mdl-26914638

RESUMO

There is great demand for high-throughput methods to characterize ligand affinity. By combining mRNA display with next-generation sequencing, we determined the kinetic on- and off-rates for over twenty thousand ligands without the need for synthesis or purification of individual members. Our results are reproducible and as accurate as those obtained with other methods of affinity measurement.

Assuntos

Sequenciamento de Nucleotídeos em Larga Escala; RNA Mensageiro/genética; Cinética; Ligantes

Palavras-chave

affinity measurements; dissociation constants; high-throughput screenings; kinetics; protein-protein interactions

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Sequenciamento de Nucleotídeos em Larga Escala Idioma: En Revista: Angew Chem Int Ed Engl Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Estados Unidos

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