Characterization at the individual cell level and in whole blood samples of shear stress preventing red blood cells aggregation.
J Biomech
; 49(7): 1021-1026, 2016 05 03.
Article
em En
| MEDLINE
| ID: mdl-26916508
ABSTRACT
The aggregation of red blood cells (RBC) is an intrinsic feature of blood that has a strong impact on its microcirculation. For a number of years it has been attracting a great attention in basic research and clinical studies. Here, we study a relationship between the RBC aggregation parameters measured at the individual cell level and in a whole blood sample. The home made optical tweezers were used to measure the aggregating and disaggregating forces for a pair of interacting RBCs, at the individual cell level, in order to evaluate the corresponding shear stresses. The RheoScan aggregometer was used for the measurements of critical shear stress (CSS) in whole blood samples. The correlation between CSS and the shear stress required to stop an RBC pair from aggregating was found. The shear stress required to disaggregate a pair of RBCs using the double channel optical tweezers appeared to be about 10 times higher than CSS. The correlation between shear stresses required to prevent RBCs from aggregation at the individual cell level and in whole blood samples was estimated and assessed quantitatively. The experimental approach developed has a high potential for advancing hemorheological studies.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Agregação Eritrocítica
/
Eritrócitos
Limite:
Adult
/
Humans
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Male
Idioma:
En
Revista:
J Biomech
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Finlândia