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Clathrin regulates blue light-triggered lateral auxin distribution and hypocotyl phototropism in Arabidopsis.
Zhang, Ying; Yu, Qinqin; Jiang, Nan; Yan, Xu; Wang, Chao; Wang, Qingmei; Liu, Jianzhong; Zhu, Muyuan; Bednarek, Sebastian Y; Xu, Jian; Pan, Jianwei.
Afiliação
  • Zhang Y; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, China.
  • Yu Q; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, China.
  • Jiang N; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, China.
  • Yan X; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, China.
  • Wang C; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, China.
  • Wang Q; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, China.
  • Liu J; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, China.
  • Zhu M; Institute of Genetics, Key Laboratory for Cell and Gene Engineering of Zhejiang Province, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
  • Bednarek SY; Department of Biochemistry, University of Wisconsin - Madison, Madison, WI, 53706, USA.
  • Xu J; Department of Biological Sciences and NUS Centre for BioImaging Sciences, National University of Singapore, 14 Science Drive 4, 117543, Singapore.
  • Pan J; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, China.
Plant Cell Environ ; 40(1): 165-176, 2017 01.
Article em En | MEDLINE | ID: mdl-27770560
Phototropism is the process by which plants grow towards light in order to maximize the capture of light for photosynthesis, which is particularly important for germinating seedlings. In Arabidopsis, hypocotyl phototropism is predominantly triggered by blue light (BL), which has a profound effect on the establishment of asymmetric auxin distribution, essential for hypocotyl phototropism. Two auxin efflux transporters ATP-binding cassette B19 (ABCB19) and PIN-formed 3 (PIN3) are known to mediate the effect of BL on auxin distribution in the hypocotyl, but the details for how BL triggers PIN3 lateralization remain poorly understood. Here, we report a critical role for clathrin in BL-triggered, PIN3-mediated asymmetric auxin distribution in hypocotyl phototropism. We show that unilateral BL induces relocalization of clathrin in the hypocotyl. Loss of clathrin light chain 2 (CLC2) and CLC3 affects endocytosis and lateral distribution of PIN3 thereby impairing BL-triggered establishment of asymmetric auxin distribution and consequently, phototropic bending. Conversely, auxin efflux inhibitors N-1-naphthylphthalamic acid and 2,3,5-triiodobenzoic acid affect BL-induced relocalization of clathrin, endocytosis and lateralization of PIN3 as well as asymmetric distribution of auxin. These results together demonstrate an important interplay between auxin and clathrin function that dynamically regulates BL-triggered hypocotyl phototropism in Arabidopsis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Clatrina / Arabidopsis / Fototropismo / Hipocótilo / Ácidos Indolacéticos / Luz Idioma: En Revista: Plant Cell Environ Assunto da revista: BOTANICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Clatrina / Arabidopsis / Fototropismo / Hipocótilo / Ácidos Indolacéticos / Luz Idioma: En Revista: Plant Cell Environ Assunto da revista: BOTANICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China