Cryogenic optical localization provides 3D protein structure data with Angstrom resolution.
Nat Methods
; 14(2): 141-144, 2017 02.
Article
em En
| MEDLINE
| ID: mdl-28068317
ABSTRACT
We introduce Cryogenic Optical Localization in 3D (COLD), a method to localize multiple fluorescent sites within a single small protein with Angstrom resolution. We demonstrate COLD by determining the conformational state of the cytosolic Per-ARNT-Sim domain from the histidine kinase CitA of Geobacillus thermodenitrificans and resolving the four biotin sites of streptavidin. COLD provides quantitative 3D information about small- to medium-sized biomolecules on the Angstrom scale and complements other techniques in structural biology.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Óptica e Fotônica
/
Corantes Fluorescentes
/
Histidina Quinase
/
Imagem Individual de Molécula
/
Microscopia de Fluorescência
Idioma:
En
Revista:
Nat Methods
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Alemanha