Durable vesicles for reconstitution of membrane proteins in biotechnology.
Biochem Soc Trans
; 45(1): 15-26, 2017 Feb 08.
Article
em En
| MEDLINE
| ID: mdl-28202656
ABSTRACT
The application of membrane proteins in biotechnology requires robust, durable reconstitution systems that enhance their stability and support their functionality in a range of working environments. Vesicular architectures are highly desirable to provide the compartmentalisation to utilise the functional transmembrane transport and signalling properties of membrane proteins. Proteoliposomes provide a native-like membrane environment to support membrane protein function, but can lack the required chemical and physical stability. Amphiphilic block copolymers can also self-assemble into polymersomes tough vesicles with improved stability compared with liposomes. This review discusses the reconstitution of membrane proteins into polymersomes and the more recent development of hybrid vesicles, which blend the robust nature of block copolymers with the biofunctionality of lipids. These novel synthetic vesicles hold great promise for enabling membrane proteins within biotechnologies by supporting their enhanced in vitro performance and could also contribute to fundamental biochemical and biophysical research by improving the stability of membrane proteins that are challenging to work with.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteolipídeos
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Biotecnologia
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Lipossomas Unilamelares
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Bicamadas Lipídicas
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Proteínas de Membrana
Idioma:
En
Revista:
Biochem Soc Trans
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Reino Unido