cCMP and cUMP in Apoptosis: Concepts and Methods.
Handb Exp Pharmacol
; 238: 25-47, 2017.
Article
em En
| MEDLINE
| ID: mdl-28456891
ABSTRACT
The cyclic nucleotides cAMP and cGMP are well-characterized second messenger molecules regulating many important intracellular processes, such as differentiation, proliferation, and apoptosis. The latter is a highly regulated process of programmed cell death wherein several regulatory proteins, like those belonging to the Bcl-2 family, are involved. The initiation of apoptosis is regulated by three different pathways the intrinsic or mitochondrial, the extrinsic, and the ER stress pathway. Recently, it has been published that the pyrimidine cyclic nucleotides cCMP and cUMP also function as second messenger molecules, and additionally have an effect on apoptosis signaling pathways. cCMP induced PKA-independent apoptosis via the intrinsic and ER-stress pathway in S49 mouse lymphoma cells, and cCMP as well as cUMP induced apoptosis in human HEL cells via the intrinsic pathway. However, in human K-562 cells, which are known to be multidrug-resistant, cCMP and cUMP had no effect. Summarized in this chapter are the initiation of apoptosis by cCMP and cUMP regarding the various apoptotic pathways, the enzymes involved in apoptosis, as well as the most relevant methods for the detection and examination of apoptosis and the corresponding signaling pathways.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Uridina Monofosfato
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Bioensaio
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Sistemas do Segundo Mensageiro
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Apoptose
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CMP Cíclico
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Nucleotídeos Cíclicos
Limite:
Animals
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Humans
Idioma:
En
Revista:
Handb Exp Pharmacol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Alemanha