A DiCre recombinase-based system for inducible expression in Leishmania major.
Mol Biochem Parasitol
; 216: 45-48, 2017 09.
Article
em En
| MEDLINE
| ID: mdl-28629935
ABSTRACT
Here we present the establishment of an inducible system based on the dimerizable Cre recombinase (DiCre) for controlled gene expression in the protozoan parasite Leishmania. Rapamycin-induced DiCre activation promoted efficient flipping and expression of gene products in a time and dose-dependent manner. The DiCre flipping activity induced the expression of target genes from both integrated and episomal contexts broadening the applicability of the system. We validated the system by inducing the expression of both full length and truncated forms of the checkpoint protein Rad9, which revealed that the highly divergent C-terminal domain of Rad9 is necessary for proper subcellular localization. Thus, by establishing the DiCre-based inducible system we have created and validated a robust new tool for assessing gene function in Leishmania.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Engenharia Genética
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Regulação da Expressão Gênica
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Leishmania major
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Integrases
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Recombinação Homóloga
Idioma:
En
Revista:
Mol Biochem Parasitol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Brasil