[Preparation of CD123 mono-antibody modified tanshinone â
¡A loaded immunoliposome and its in vitro evaluation].
Zhongguo Zhong Yao Za Zhi
; 42(11): 2085-2091, 2017 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-28822152
ABSTRACT
In the study, we developed a novel formulation, CD123 mono-antibody (mAb) modified tanshinone â
¡A loaded immunoliposome (CD123-Tanâ
¡A-ILP) to achieve the targeted drug delivery for leukemia cells. Orthogonal test was used to optimize liposome preparation, and the Tanâ
¡A-loaded PEGylated liposomes (Tanâ
¡A-LP) of S100PC-Chol-(mPEG2000-DSPE)-Tanâ
¡A at 19â¶5â¶1â¶1 molar ratio were prepared by the thin film hydration-probe ultrasonic method. A post-insertion method was applied to prepare CD123-Tanâ
¡A-ILP via thiolated mAb conjugated to the terminal of maleimide-PEG2000-DSPE. The cellular uptake assay was measured by flow cytometry, and the inhibitory effect of CD123-Tanâ
¡A-ILP on NB4 cells proliferation was tested by using MTT assay. The results of cellular uptake assay showed that CD123-ILP could significantly increase the drug uptake of NB4 cells as compared with free drugs and LP. The IC50 values at 48 h incubation were 20.87, 11.71, 7.17 µmolâ¢L⻹ respectively for Tanâ
¡Aï¼Tanâ
¡A-LP and CD123-Tanâ
¡A-ILP. CD123-ILP demonstrated a potential and promising targeted drug delivery strategy for acute myelogenous leukemia (AML) treatment.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sistemas de Liberação de Medicamentos
/
Abietanos
/
Lipossomos
/
Anticorpos Monoclonais
/
Antineoplásicos Fitogênicos
Limite:
Humans
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Assunto da revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China