Affinity resins as new tools for identifying target proteins of ascorbic acid.
Analyst
; 143(4): 874-882, 2018 Feb 12.
Article
em En
| MEDLINE
| ID: mdl-29327754
ABSTRACT
l-Ascorbic acid (AA) has diverse physiological functions, but little is known about the functional mechanisms of AA. In this study, we synthesized two types of affinity resin on which AA is immobilized in a stable form to identify new AA-targeted proteins, which can provide important clues for elucidating unknown functional mechanisms of AA. To our knowledge, an affinity resin on which AA as a ligand is immobilized has not been prepared, because AA is very unstable and rapidly degraded in an aqueous solution. By using the affinity resins, cytochrome c (cyt c) was identified as an AA-targeted protein, and we showed that oxidized cyt c exhibits specific affinity for AA. These results suggest that two kinds of AA-affinity resin can be powerful tools to identify new target proteins of AA.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácido Ascórbico
/
Proteínas
/
Cromatografia de Afinidade
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Analyst
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Japão