Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells.

Harikumar, Kaleeckal G; Yan, Yan; Xu, Ting-Hai; Melcher, Karsten; Xu, H Eric; Miller, Laurence J

Harikumar, Kaleeckal G; Yan, Yan; Xu, Ting-Hai; Melcher, Karsten; Xu, H Eric; Miller, Laurence J.

Afiliação

Harikumar KG; Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA.
Yan Y; Key Laboratory of Receptor Research, VARI-SIMM Center, Center for Structure and Function of Drug Targets, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Xu TH; University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing 100049, China.
Melcher K; Center for Cancer and Cell Biology, Innovation and Integration Program, Van Andel Research Institute, 333 Bostwick Avenue Northeast, Grand Rapids, MI 49503, USA.
Xu HE; Key Laboratory of Receptor Research, VARI-SIMM Center, Center for Structure and Function of Drug Targets, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Miller LJ; University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing 100049, China.

Bio Protoc ; 7(22)2017 Nov 20.

Article em En | MEDLINE | ID: mdl-29423426

Palavras-chave

BRET assay; Coelenterazine h; Molecular approximation/interaction; Renilla luciferase; Yellow fluorescent protein

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Bio Protoc Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos

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