A Structured Workflow for Mapping Human Sin3 Histone Deacetylase Complex Interactions Using Halo-MudPIT Affinity-Purification Mass Spectrometry.

Banks, Charles A S; Thornton, Janet L; Eubanks, Cassandra G; Adams, Mark K; Miah, Sayem; Boanca, Gina; Liu, Xingyu; Katt, Maria L; Parmely, Tari J; Florens, Laurence; Washburn, Michael P

Banks, Charles A S; Thornton, Janet L; Eubanks, Cassandra G; Adams, Mark K; Miah, Sayem; Boanca, Gina; Liu, Xingyu; Katt, Maria L; Parmely, Tari J; Florens, Laurence; Washburn, Michael P.

Afiliação

Banks CAS; From the Stowers Institute for Medical Research, Kansas City, MO 64110.
Thornton JL; From the Stowers Institute for Medical Research, Kansas City, MO 64110.
Eubanks CG; From the Stowers Institute for Medical Research, Kansas City, MO 64110.
Adams MK; From the Stowers Institute for Medical Research, Kansas City, MO 64110.
Miah S; From the Stowers Institute for Medical Research, Kansas City, MO 64110.
Boanca G; From the Stowers Institute for Medical Research, Kansas City, MO 64110.
Liu X; From the Stowers Institute for Medical Research, Kansas City, MO 64110.
Katt ML; From the Stowers Institute for Medical Research, Kansas City, MO 64110.
Parmely TJ; From the Stowers Institute for Medical Research, Kansas City, MO 64110.
Florens L; From the Stowers Institute for Medical Research, Kansas City, MO 64110.
Washburn MP; From the Stowers Institute for Medical Research, Kansas City, MO 64110; mpw@stowers.org.

Mol Cell Proteomics ; 17(7): 1432-1447, 2018 07.

Article em En | MEDLINE | ID: mdl-29599190

Assuntos

Cromatografia de Afinidade/métodos; Espectrometria de Massas/métodos; Mapeamento de Interação de Proteínas/métodos; Complexo Correpressor Histona Desacetilase e Sin3/metabolismo; Fluxo de Trabalho; Linhagem Celular; Células HEK293; Humanos; Ligação Proteica; Subunidades Proteicas/metabolismo

Palavras-chave

Affinity tagging; Chromatin function or biology; Imaging; Label-free quantification; Macromolecular complex analysis; Networks*; Protein complex analysis; Protein-Protein Interactions*

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Cromatografia de Afinidade / Mapeamento de Interação de Proteínas / Complexo Correpressor Histona Desacetilase e Sin3 / Fluxo de Trabalho Limite: Humans Idioma: En Revista: Mol Cell Proteomics Assunto da revista: BIOLOGIA MOLECULAR / BIOQUIMICA Ano de publicação: 2018 Tipo de documento: Article

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