hst-1 transforming protein: expression in silkworm cells and characterization as a novel heparin-binding growth factor.
Oncogene
; 3(4): 383-9, 1988 Oct.
Article
em En
| MEDLINE
| ID: mdl-2978866
ABSTRACT
A protein encoded by an hst-1 transforming gene was expressed in silkworm-derived BmN cells and secreted into the culture medium using a recombinant baculovirus vector. The strong affinity for heparin of the secreted protein made it possible to purify the hst-1 protein to homogeneity in a two-step procedure. The purified hst-1 protein has a molecular weight of 18,000 and stimulates both DNA synthesis in NIH3T3 cells and human umbilical vein endothelial cell proliferation. In addition, morphological changes and anchorage-independent growth of NIH3T3 cells are induced by this product. These results show that the hst-1 transforming protein is a novel heparin-binding growth factor as predicted by nucleotide sequence analysis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oncogenes
/
Heparina
/
Proteínas Proto-Oncogênicas
/
Substâncias de Crescimento
/
Proteínas Oncogênicas
/
Fatores de Crescimento de Fibroblastos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Oncogene
Assunto da revista:
BIOLOGIA MOLECULAR
/
NEOPLASIAS
Ano de publicação:
1988
Tipo de documento:
Article
País de afiliação:
Japão