Investigating pediatric disorders with induced pluripotent stem cells.
Pediatr Res
; 84(4): 499-508, 2018 10.
Article
em En
| MEDLINE
| ID: mdl-30065271
ABSTRACT
The study of disease pathophysiology has long relied on model systems, including animal models and cultured cells. In 2006, Shinya Yamanaka achieved a breakthrough by reprogramming somatic cells into induced pluripotent stem cells (iPSCs). This revolutionary discovery provided new opportunities for disease modeling and therapeutic intervention. With established protocols, investigators can generate iPSC lines from patient blood, urine, and tissue samples. These iPSCs retain ability to differentiate into every human cell type. Advances in differentiation and organogenesis move cellular in vitro modeling to a multicellular model capable of recapitulating physiology and disease. Here, we discuss limitations of traditional animal and tissue culture models, as well as the application of iPSC models. We highlight various techniques, including reprogramming strategies, directed differentiation, tissue engineering, organoid developments, and genome editing. We extensively summarize current established iPSC disease models that utilize these techniques. Confluence of these technologies will advance our understanding of pediatric diseases and help usher in new personalized therapies for patients.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pediatria
/
Pesquisa Biomédica
/
Células-Tronco Pluripotentes Induzidas
Tipo de estudo:
Guideline
Limite:
Animals
/
Child
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Humans
Idioma:
En
Revista:
Pediatr Res
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Estados Unidos