Organization of genes encoding two outer membrane proteins of the Lyme disease agent Borrelia burgdorferi within a single transcriptional unit.
Infect Immun
; 54(1): 207-12, 1986 Oct.
Article
em En
| MEDLINE
| ID: mdl-3019889
ABSTRACT
OspA and OspB are major outer membrane proteins and antigens of the Lyme disease spirochete, Borrelia burgdorferi. We examined the organization of ospA and ospB, the genes encoding these proteins. The location and direction of transcription of each osp gene was determined by subcloning, deletion analysis, and transposon Tn5 mutagenesis. Transposon Tn5 insertions within the ospA gene abrogated expression of ospB, suggesting that these genes are transcribed from a common promoter. Northern blot analysis of mRNA from B. burgdorferi with two DNA probes individually specific for ospA or ospB identified a 2.2-kilobase transcript that hybridized with each probe. These studies indicate that the two osp genes of B. burgdorferi constitute a single transcriptional unit.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas da Membrana Bacteriana Externa
/
Borrelia
/
Genes Bacterianos
Idioma:
En
Revista:
Infect Immun
Ano de publicação:
1986
Tipo de documento:
Article