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High-depth fluorescence imaging using a two-photon FRET system for mitochondrial pH in live cells and tissues.
Chang, Min Jung; Kim, Kyutae; Park, Kyun Seob; Kang, Ji Su; Lim, Chang Su; Kim, Hwan Myung; Kang, Chulhun; Lee, Min Hee.
Afiliação
  • Chang MJ; Department of Chemistry, Sookmyung Women's University, Seoul, 04310, Korea. minheelee@sookmyung.ac.kr.
  • Kim K; The School of East-West Medical Science, Kyung Hee University, Yongin, 17104, Korea. kangch@khu.ac.kr.
  • Park KS; The School of East-West Medical Science, Kyung Hee University, Yongin, 17104, Korea. kangch@khu.ac.kr.
  • Kang JS; Department of Chemistry, Ajou University, Suwon, 16499, Korea. cslim8112@gmail.com kimhm@ajou.ac.kr.
  • Lim CS; Department of Chemistry, Ajou University, Suwon, 16499, Korea. cslim8112@gmail.com kimhm@ajou.ac.kr.
  • Kim HM; Department of Chemistry, Ajou University, Suwon, 16499, Korea. cslim8112@gmail.com kimhm@ajou.ac.kr.
  • Kang C; The School of East-West Medical Science, Kyung Hee University, Yongin, 17104, Korea. kangch@khu.ac.kr.
  • Lee MH; Department of Chemistry, Sookmyung Women's University, Seoul, 04310, Korea. minheelee@sookmyung.ac.kr.
Chem Commun (Camb) ; 54(96): 13531-13534, 2018 Dec 14.
Article em En | MEDLINE | ID: mdl-30431633
ABSTRACT
We developed a fluorescent pH probe (1) capable of two-photon excitation and far-visible-emission based on FRET, composed of naphthalimide-piperazine-rhodamine. It exhibited a pH-dependent reversible and fast ratiometric fluorescence change in the rhodamine emission. Probe 1 was applied to image the pH perturbations of mitochondria in living cells and tissues.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Piperazinas / Rodaminas / Naftalimidas / Corantes Fluorescentes / Mitocôndrias Limite: Humans Idioma: En Revista: Chem Commun (Camb) Assunto da revista: QUIMICA Ano de publicação: 2018 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Piperazinas / Rodaminas / Naftalimidas / Corantes Fluorescentes / Mitocôndrias Limite: Humans Idioma: En Revista: Chem Commun (Camb) Assunto da revista: QUIMICA Ano de publicação: 2018 Tipo de documento: Article