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[Mass spectrometry guided strategy based on feature fragment ions for guided-separation on quinoline alkaloids from root barks of Dictamnus dasycarpus].
Guo, Xi-Xi; Bi, Qi-Rui; Wang, Zhe; Tan, Ning-Hua.
Afiliação
  • Guo XX; School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China.
  • Bi QR; School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China.
  • Wang Z; School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China.
  • Tan NH; School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China.
Zhongguo Zhong Yao Za Zhi ; 43(19): 3887-3892, 2018 Oct.
Article em Zh | MEDLINE | ID: mdl-30453714
The root bark of Dictamnus dasycarpus is one of common traditional Chinese medicines (TCMs). Quinoline alkaloids are one of the main active substances in this TCM and possess many biological activities including anti-titumor, anti-inflammation, anti-bacteria, anti-oxidation, and anti-platelet aggregation activities. In this study, eight quinoline alkaloids 1-8 were firstly separated from the root barks of D. dasycarpus. It was difficult to isolate more quinoline alkaloids from the remaining fraction 8 in D. dasycarpus by this conventional chemical separation, so the target analysis method combined LC-MS guided-separation of quinoline alkaloids from fraction 8 was established. MS/MS fragmentation patterns of eight quinoline alkaloids reference standard compounds 1-8 were studied by ultra-performance liquid chromatography-electrospary ionization-mass spectrometry (UPLC-ESI-MS/MS). Based on the feature fragment ion m/z 200, the parent ion scan mode was established for the target analysis of quinoline alkaloids in fraction 8. Finally, 8-methoxyflindersine (9) and N-metilatanina (10) were discovered and isolated quickly from fraction 8 guided by LC-MS, and their structures were identified by NMR and MS. Among them, compound 10 was isolated from the genus Dictamnus for the first time. These results indicated that this method is not only quick and sensitive for analyzing the quinoline alkaloids, but also to effectively guided-separate this kind of alkaloids in the root barks of D. dasycarpus.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quinolinas / Dictamnus / Alcaloides Idioma: Zh Revista: Zhongguo Zhong Yao Za Zhi Assunto da revista: FARMACOLOGIA / TERAPIAS COMPLEMENTARES Ano de publicação: 2018 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quinolinas / Dictamnus / Alcaloides Idioma: Zh Revista: Zhongguo Zhong Yao Za Zhi Assunto da revista: FARMACOLOGIA / TERAPIAS COMPLEMENTARES Ano de publicação: 2018 Tipo de documento: Article País de afiliação: China