Detection of IL-1ß, IL-6 and TNF-α in Sprague-Dawely rats' atrophic thymus induced by lipopolysaccharide.
Pol J Vet Sci
; 21(3): 589-597, 2018 Sep.
Article
em En
| MEDLINE
| ID: mdl-30468342
ABSTRACT
OBJECTIVE:
This study aimed to investigate developmental changes of the thymus and intra- thymic IL-1ß, IL-6 and TNF-α expression in weaned Sprague-Dawley rats induced by lipopolysac- charide.METHODS:
Forty healthy weaned rats aged 26 days and weighing 83±4 g were randomly and equally divided into two groups. The lipopolysaccharide group was treated daily with a single injection of lipopolysaccharide for 10 consecutive days, and the saline group was treated with an equal volume of sterilized saline. On the 1st, 4th, 7th and 10th day, histological changes and distribu- tion of IL-1ß-, IL-6- and TNF-α-positive cells were detected in the thymus by hematoxylin-eosin and immunohistochemistry staining, respectively. Subsequently, the expression levels of IL-1ß, IL-6 and TNF-α were evaluated in the thymus by the ELISA method.RESULTS:
Thymus weight and index were significantly smaller in lipopolysaccharide-treated rats than in saline-treated rats (p⟨0.05), but no substantial changes were found in the thymus microstructure after lipopolysaccharide induction. Moreover, a large number of IL-1ß-, IL-6- and TNF-α-positive cells were observed with brownish-yellow color and mainly distributed in the thy- mus parenchyma, both integrated optical density and average optical density increased signifi- cantly in lipopolysaccharide-treated rats than those in saline-treated rats. Compared with the saline group, most of the thymic homogenates had higher levels of IL-1ß, IL-6 and TNF-α in the lipopolysaccharide group on different days.CONCLUSION:
These findings indicate that the thymus atrophied after lipopolysaccharide induction in weaned Sprague-Dawley rats, and excessive production of intrathymic IL-1ß, IL-6 and TNF-α was probably involved in the atrophic process.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Timo
/
Lipopolissacarídeos
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Interleucina-6
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Fator de Necrose Tumoral alfa
/
Interleucina-1beta
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Pol J Vet Sci
Assunto da revista:
MEDICINA VETERINARIA
Ano de publicação:
2018
Tipo de documento:
Article