Highly efficient scarless knock-in of reporter genes into human and mouse pluripotent stem cells via transient antibiotic selection.

Sluch, Valentin M; Chamling, Xitiz; Wenger, Claire; Duan, Yukan; Rice, Dennis S; Zack, Donald J

Sluch, Valentin M; Chamling, Xitiz; Wenger, Claire; Duan, Yukan; Rice, Dennis S; Zack, Donald J.

Afiliação

Sluch VM; Department of Ophthalmology, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, United States of America.
Chamling X; Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.
Wenger C; McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine Baltimore, Maryland, United States of America.
Duan Y; Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.
Rice DS; Department of Ophthalmology, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, United States of America.
Zack DJ; Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.

PLoS One ; 13(11): e0201683, 2018.

Article em En | MEDLINE | ID: mdl-30496180

Assuntos

Técnicas de Introdução de Genes/métodos; Células-Tronco Pluripotentes/metabolismo; Animais; Linhagem Celular; Genes Reporter; Humanos; Proteínas Luminescentes/biossíntese; Proteínas Luminescentes/genética; Camundongos; Células-Tronco Pluripotentes/citologia; Transgenes

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes / Técnicas de Introdução de Genes Limite: Animals / Humans Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Estados Unidos

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