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Identification of Methylated Transcripts Using the TRIBE Approach.
Worpenberg, Lina; Jakobi, Tobias; Dieterich, Christoph; Roignant, Jean-Yves.
Afiliação
  • Worpenberg L; Laboratory of RNA Epigenetics, Institute of Molecular Biology (IMB), Mainz, Germany.
  • Jakobi T; Department of Internal Medicine III, University Hospital Heidelberg, Heidelberg, Germany.
  • Dieterich C; DZHK (German Centre for Cardiovascular Research), Partner Site Heidelberg/Mannheim, Heidelberg, Germany.
  • Roignant JY; Department of Internal Medicine III, University Hospital Heidelberg, Heidelberg, Germany. christoph.dieterich@uni-heidelberg.de.
Methods Mol Biol ; 1870: 89-106, 2019.
Article em En | MEDLINE | ID: mdl-30539549
m6A is the most abundant internal modification on mRNA. Recent improvements of high-throughput sequencing techniques enables its detection at the transcriptome level, even at the nucleotide resolution. However most current techniques require large amounts of starting material to detect the modification. Here, we describe a complementary technique of standard meRIP-seq/miCLIP-seq approaches to identify methylated RNA using a low amount of material. We believe this approach can be applied in vivo to identify methylated targets in specific tissues or subpopulations of cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Biologia Computacional Tipo de estudo: Diagnostic_studies Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Biologia Computacional Tipo de estudo: Diagnostic_studies Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Alemanha