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Expression, purification, crystallization and preliminary X-ray diffraction analysis of swine leukocyte antigen 2 complexed with a CTL epitope AS64 derived from Asia1 serotype of foot-and-mouth disease virus.
Gao, Feng-Shan; Feng, Lei; Jiang, Ping; Li, Zi-Bin; Gao, Hua; Zhai, Xiao-Xin; Zhang, Zong-Hui; Hu, Xiao.
Afiliação
  • Gao FS; Department of Bioengineering, |College of Life Science and Technology, Dalian University, Xuefu street 10, Dalian, Liaoning, 116622, People's Republic of China. gfsh0626@126.com.
  • Feng L; Department of Bioengineering, |College of Life Science and Technology, Dalian University, Xuefu street 10, Dalian, Liaoning, 116622, People's Republic of China.
  • Jiang P; Department of Bioengineering, |College of Life Science and Technology, Dalian University, Xuefu street 10, Dalian, Liaoning, 116622, People's Republic of China.
  • Li ZB; Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing, 100094, People's Republic of China.
  • Gao H; Department of Bioengineering, |College of Life Science and Technology, Dalian University, Xuefu street 10, Dalian, Liaoning, 116622, People's Republic of China.
  • Zhai XX; Department of Bioengineering, |College of Life Science and Technology, Dalian University, Xuefu street 10, Dalian, Liaoning, 116622, People's Republic of China.
  • Zhang ZH; Department of Bioengineering, |College of Life Science and Technology, Dalian University, Xuefu street 10, Dalian, Liaoning, 116622, People's Republic of China.
  • Hu X; Department of Bioengineering, |College of Life Science and Technology, Dalian University, Xuefu street 10, Dalian, Liaoning, 116622, People's Republic of China.
BMC Vet Res ; 14(1): 407, 2018 Dec 18.
Article em En | MEDLINE | ID: mdl-30563524
BACKGROUND: Currently, the structural characteristics of the swine major histocompatibility complex (MHC) class I molecule, also named swine leukocyte antigen class I (SLA-I) molecule need to be further clarified. RESULTS: A complex of SLA-I constituted by an SLA-2*HB01 molecule with swine ß2-microglobulin and a cytotoxic T lymphocyte (CTL) epitope FMDV-AS64 (ALLRSATYY) derived from VP1 protein (residues 64-72) of Asia 1 serotype of foot-and-mouth disease virus (FMDV) was expressed, refolded, purified and crystallized. By preliminary X-ray diffraction analysis, it was shown that the diffraction resolution of the crystal was 2.4 Å and the space group belonged to P212121 with unit cell parameters a = 48.37, b = 97.75, c = 166.163 Å. CONCLUSION: This research will be in favor of illuminating the structural characteristics of an SLA-2 molecule associated with a CTL epitope derived from Asia1 serotype of FMDV.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Difração de Raios X / Antígenos de Histocompatibilidade Classe I / Modelos Moleculares / Vírus da Febre Aftosa / Epitopos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: BMC Vet Res Assunto da revista: MEDICINA VETERINARIA Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Difração de Raios X / Antígenos de Histocompatibilidade Classe I / Modelos Moleculares / Vírus da Febre Aftosa / Epitopos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: BMC Vet Res Assunto da revista: MEDICINA VETERINARIA Ano de publicação: 2018 Tipo de documento: Article