Cloning of the HhaI and HinPI restriction-modification systems.
Gene
; 74(1): 5-7, 1988 Dec 25.
Article
em En
| MEDLINE
| ID: mdl-3074018
ABSTRACT
The genes for the HhaI (Roberts et al., 1976) and HinPI (Roberts, 1987) restriction-modification (R-M) systems have been cloned in pBR322. The HhaI system was isolated on a 9-kb PstI fragment, and the HinPI system was isolated on two PstI fragments of 1.5 and 4.6 kb in length. The clones were isolated by selecting for recombinant molecules that had protectively modified themselves. The HhaI and HinPI R-M systems recognize the same sequence, GCGC, but hybridization between the DNA fragments encoding them does not take place.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Desoxirribonucleases de Sítio Específico do Tipo II
/
DNA-Citosina Metilases
/
Haemophilus
/
Haemophilus influenzae
Idioma:
En
Revista:
Gene
Ano de publicação:
1988
Tipo de documento:
Article