Styrene-N-phenylacrylamide co-polymer modified silica monolith particles with an optimized mixing ratio of monomers as a new stationary phase for the separation of peptides in high performance liquid chromatography.
J Sep Sci
; 42(16): 2612-2620, 2019 Aug.
Article
em En
| MEDLINE
| ID: mdl-31161715
ABSTRACT
A stationary phase was prepared by chemical derivatization of the support particles with a layer of copolymer composed of styrene and N-phenyl acrylamide. Silica monolith particles of ca. 2.6 µm (volume-based average) have been prepared as the support particles by sol-gel reaction followed by differential sedimentation. The particles were reacted with 3-chloropropyl trimethoxysilane followed by sodium diethyldithiocarbamate to introduce an initiator moiety. Then, the copolymer layer was immobilized via reversible addition-fragmentation transfer polymerization. The resultant phase was packed in glass-lined stainless-steel micro-columns (1 x 150 mm) and evaluated for the separation of a mixture composed of five peptides (Trp-Gly, Thr-Tyr-Ser, angiotensin I, isotocin and bradykinin). The effect of monomer mixing ratio (styrene versus N-phenyl acrylamide) on the chromatographic separation efficiency of the stationary phase was examined. A number of theoretical plates (N) as high as 33 600 plates/column (224 000 plates/m, 4.46 µm plate height) was achieved using the column packed with the optimized stationary phase. The column-to-column reproducibility based on three columns packed with three different batches of stationary phase was found satisfactory in separation efficiency, retention factor, and asymmetry factor.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
J Sep Sci
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Coréia do Sul