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One immunoassay probe makes SERS and fluorescence two readout signals: Rapid imaging and determination of intracellular glutathione levels.
Yao, Wenhuan; Chang, Lu; Yin, Weidong; Wang, Tao; Yang, Yuan; Yin, Pei; Yang, Manyi; Ma, Yucheng; Qin, Yanfang; Ma, Hengchang.
Afiliação
  • Yao W; College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China. Electronic address: whyao@nwnu.edu.cn.
  • Chang L; College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
  • Yin W; College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
  • Wang T; College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
  • Yang Y; College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
  • Yin P; College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
  • Yang M; College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
  • Ma Y; College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
  • Qin Y; College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
  • Ma H; College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China. Electronic address: mahczju@hotmail.com.
Spectrochim Acta A Mol Biomol Spectrosc ; 223: 117303, 2019 Dec 05.
Article em En | MEDLINE | ID: mdl-31255857
ABSTRACT
In this paper, one probe (TPPA-VCh) with fluorescence and Surface-enhanced Raman Scattering (SERS) two readout signals, which has high sensitivity and specificity to glutathione in both vitro and cell image applications, is designed and synthesized. Furthermore, the quenched emissions and intensified SERS signals is obtained by loading TPPA-VCh on the surfaces of gold nanoparticles.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Análise Espectral Raman / Imunoensaio / Sondas Moleculares / Espaço Intracelular / Imagem Molecular / Glutationa Limite: Humans Idioma: En Revista: Spectrochim Acta A Mol Biomol Spectrosc Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2019 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Análise Espectral Raman / Imunoensaio / Sondas Moleculares / Espaço Intracelular / Imagem Molecular / Glutationa Limite: Humans Idioma: En Revista: Spectrochim Acta A Mol Biomol Spectrosc Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2019 Tipo de documento: Article