Effects of sulfanilamide on boar sperm quality, bacterial composition, and fertility during liquid storage at 17°C.
Anim Sci J
; 90(9): 1161-1169, 2019 Sep.
Article
em En
| MEDLINE
| ID: mdl-31381235
ABSTRACT
Sulfanilamide (SA) is an effective broad-spectrum antibacterial agent in human and veterinary medicine. The purpose of this study was to evaluate the effects of SA on boar sperm quality during liquid storage at 17°C and determine the optimal concentration of SA and its effects on bacterial growth, microbial composition, and maternal fertility. Boar ejaculates were diluted with a basic extender, containing different concentrations of SA, and stored in a 17°C incubator for 6 days. The sperm motility, plasma membrane integrity, and acrosome integrity were measured daily. The results showed that when the concentration of SA was 0.02 g/L, the sperm quality parameters were significantly higher than those of all other treatment groups (p < .05). We also monitored the bacterial growth and compared the differences in the microbial species between the 0.02 g/L SA group and the control by 16S rDNA sequencing. The results revealed that some bacteria, such as Staphylococcus and Pseudomonas, were considerably lower in the 0.02 g/L SA group than in the control group (p < .05). In addition, preserved semen was used for artificial insemination, and results showed that 0.02 g/L SA group had a higher litter size, and its pregnancy rate was 92.5%.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Preservação do Sêmen
/
Análise do Sêmen
/
Sulfanilamida
Limite:
Animals
Idioma:
En
Revista:
Anim Sci J
Assunto da revista:
MEDICINA VETERINARIA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China