[The value of multi-gene testing in benign or malign diagnosis of thyroid nodules with indeterminate cytology].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
; 54(10): 764-768, 2019 Oct 07.
Article
em Zh
| MEDLINE
| ID: mdl-31606990
ABSTRACT
Objective:
To study the diagnostic value of a multi-gene molecular testing in cytologically indeterminate thyroid nodules.Methods:
From February 2018 to September 2018, patients with thyroid nodules who underwent fine needle aspiration(FNA) at Peking University Cancer Hospital were enrolled. Three hundred and sixty patients were included, consisting of 86 men and 274 women, with a mean age of 45.8 years (between 13 and 89 years old). Among 391 nodules, 141 were cytologically inderminate and 75 were resected. FNA samples underwent prospective testing using a next-generation sequencing (NGS) assay, which included 16 genes for point mutations and 26 types of gene fusions. The testing results of indeterminate nodules were compared with surgical outcomes, to determine the diagnostic performance. The results were compared with the BRAF V600E single gene mutation analysis by χ(2) test.Results:
The multi-gene testing showed a sensitivity of 73.2%, specificity of 96.8%, positive predictive value of 96.8%, and negative predictive value of 73.2%. The diagnostic accuracy of multi-gene testing was significantly higher than the BRAF V600E mutation test (83.3% vs 73.6%, χ(2)=31.588, P<0.01).Conclusion:
Multi-gene testing in FNA samples is an effective method to diagnose cytologically indeterminate thyroid nodules, which has a higher accuracy than BRAF V600E mutation detection.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Testes Genéticos
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Nódulo da Glândula Tireoide
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Biópsia por Agulha Fina
Tipo de estudo:
Diagnostic_studies
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Observational_studies
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Prognostic_studies
/
Risk_factors_studies
Limite:
Adolescent
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Adult
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Aged
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Aged80
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Female
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Humans
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Male
/
Middle aged
Idioma:
Zh
Revista:
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China