Reactive oxygen species generating photosynthesized ferromagnetic iron oxide nanorods as promising antileishmanial agent.
Nanomedicine (Lond)
; 15(8): 755-771, 2020 04.
Article
em En
| MEDLINE
| ID: mdl-32193975
ABSTRACT
Aim:
To investigate the photodynamic therapeutic potential of ferromagnetic iron oxide nanorods (FIONs), using Trigonella foenum-graecum as a reducing agent, against Leishmania tropica. Materials &methods:
FIONs were characterized using ultraviolet visible spectroscopy, x-ray diffraction and scanning electron microscopy.Results:
FIONs showed excellent activity against L. tropica promastigotes and amastigotes (IC50 0.036 ± 0.003 and 0.072 ± 0.001 µg/ml, respectively) upon 15 min pre-incubation light-emitting diode light (84 lm/W) exposure, resulting in reactive oxygen species generation and induction of cell death via apoptosis. FIONs were found to be highly biocompatible with human erythrocytes (LD50 779 ± 21 µg/ml) and significantly selective (selectivity index >1000) against murine peritoneal macrophages (CC50 102.7 ± 2.9 µg/ml).Conclusion:
Due to their noteworthy in vitro antileishmanial properties, FIONs should be further investigated in an in vivo model of the disease.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leishmania tropica
/
Compostos Férricos
/
Espécies Reativas de Oxigênio
/
Nanotubos
/
Antiprotozoários
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Nanomedicine (Lond)
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Paquistão