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An engineered PET depolymerase to break down and recycle plastic bottles.
Tournier, V; Topham, C M; Gilles, A; David, B; Folgoas, C; Moya-Leclair, E; Kamionka, E; Desrousseaux, M-L; Texier, H; Gavalda, S; Cot, M; Guémard, E; Dalibey, M; Nomme, J; Cioci, G; Barbe, S; Chateau, M; André, I; Duquesne, S; Marty, A.
Afiliação
  • Tournier V; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.
  • Topham CM; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.
  • Gilles A; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.
  • David B; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.
  • Folgoas C; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.
  • Moya-Leclair E; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.
  • Kamionka E; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.
  • Desrousseaux ML; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.
  • Texier H; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.
  • Gavalda S; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.
  • Cot M; CRITT Bio-Industries, INSA, Toulouse, France.
  • Guémard E; Carbios, Biopôle Clermont Limagne, Saint-Beauzire, France.
  • Dalibey M; Carbios, Biopôle Clermont Limagne, Saint-Beauzire, France.
  • Nomme J; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.
  • Cioci G; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.
  • Barbe S; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.
  • Chateau M; Carbios, Biopôle Clermont Limagne, Saint-Beauzire, France.
  • André I; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France. isabelle.andre@insa-toulouse.fr.
  • Duquesne S; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France. sophie.duquesne@insa-toulouse.fr.
  • Marty A; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France. alain.marty@carbios.fr.
Nature ; 580(7802): 216-219, 2020 04.
Article em En | MEDLINE | ID: mdl-32269349
Present estimates suggest that of the 359 million tons of plastics produced annually worldwide1, 150-200 million tons accumulate in landfill or in the natural environment2. Poly(ethylene terephthalate) (PET) is the most abundant polyester plastic, with almost 70 million tons manufactured annually worldwide for use in textiles and packaging3. The main recycling process for PET, via thermomechanical means, results in a loss of mechanical properties4. Consequently, de novo synthesis is preferred and PET waste continues to accumulate. With a high ratio of aromatic terephthalate units-which reduce chain mobility-PET is a polyester that is extremely difficult to hydrolyse5. Several PET hydrolase enzymes have been reported, but show limited productivity6,7. Here we describe an improved PET hydrolase that ultimately achieves, over 10 hours, a minimum of 90 per cent PET depolymerization into monomers, with a productivity of 16.7 grams of terephthalate per litre per hour (200 grams per kilogram of PET suspension, with an enzyme concentration of 3 milligrams per gram of PET). This highly efficient, optimized enzyme outperforms all PET hydrolases reported so far, including an enzyme8,9 from the bacterium Ideonella sakaiensis strain 201-F6 (even assisted by a secondary enzyme10) and related improved variants11-14 that have attracted recent interest. We also show that biologically recycled PET exhibiting the same properties as petrochemical PET can be produced from enzymatically depolymerized PET waste, before being processed into bottles, thereby contributing towards the concept of a circular PET economy.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plásticos / Engenharia de Proteínas / Polietilenotereftalatos / Reciclagem / Hidrolases Idioma: En Revista: Nature Ano de publicação: 2020 Tipo de documento: Article País de afiliação: França

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plásticos / Engenharia de Proteínas / Polietilenotereftalatos / Reciclagem / Hidrolases Idioma: En Revista: Nature Ano de publicação: 2020 Tipo de documento: Article País de afiliação: França