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Prokaryotic Expression of the Immunoglobulin's Domains of CRTAM to Characterize a Monoclonal Antibody.
Barragan-Galvez, Juan Carlos; Gonzalez-Orozco, Maria; Hernandez-Flores, Araceli; Maravillas-Montero, Jose Luis; Chavez-Guerrero, Yedhani; Ortiz-Navarrete, Vianney.
Afiliação
  • Barragan-Galvez JC; Biomedical Sciences Program, National Autonomous University of Mexico, Circuito Interior s/n, Cd. Universitaria, Mexico City, Mexico.
  • Gonzalez-Orozco M; Department of Molecular Biomedicine, Center for Research and Advanced Studies (CINVESTAV), Av. IPN 2508. Col. San Pedro Zacatenco, Gustavo A Madero, 07360, Mexico City, Mexico.
  • Hernandez-Flores A; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA.
  • Maravillas-Montero JL; Universidad de la Sierra Sur, Miahuatlán de Porfirio Díaz, Oaxaca, Mexico.
  • Chavez-Guerrero Y; Research Support Network, Universidad Nacional Autónoma de México and Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán", Mexico City, Mexico.
  • Ortiz-Navarrete V; Department of Molecular Biomedicine, Center for Research and Advanced Studies (CINVESTAV), Av. IPN 2508. Col. San Pedro Zacatenco, Gustavo A Madero, 07360, Mexico City, Mexico.
Protein J ; 39(3): 224-231, 2020 06.
Article em En | MEDLINE | ID: mdl-32300914
Class-I restricted T cell-associated molecule (CRTAM) is a member of the immunoglobulin superfamily, and it is closely related to nectin-like protein. CRTAM is expressed in activated CD8 T cells, NKT cells, NK cells and in a subpopulation CD4 T cells. In this study, we produce as recombinant proteins, the Ig-domains of CRTAM (IgV-IgC), the IgV, and the IgC. These proteins were successfully purified in the soluble fraction only if the stalk region was included. The recombinant CRTAM recognizes its ligand nectin-like 2 in a cell-free system. We also demonstrate that the IgC domain of CRTAM is recognized by the anti-hCRTAM monoclonal antibody C8 with a 0.62 nM affinity. In conclusion, the stalk region of CRTAM provides solubility for the expression of its Ig-domains as recombinant proteins.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Imunoglobulinas / Sistema Livre de Células / Domínios de Imunoglobulina / Molécula 1 de Adesão Celular Limite: Animals / Female / Humans Idioma: En Revista: Protein J Assunto da revista: BIOQUIMICA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: México

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Imunoglobulinas / Sistema Livre de Células / Domínios de Imunoglobulina / Molécula 1 de Adesão Celular Limite: Animals / Female / Humans Idioma: En Revista: Protein J Assunto da revista: BIOQUIMICA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: México