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Early precursors and molecular determinants of tissue-resident memory CD8+ T lymphocytes revealed by single-cell RNA sequencing.
Kurd, Nadia S; He, Zhaoren; Louis, Tiani L; Milner, J Justin; Omilusik, Kyla D; Jin, Wenhao; Tsai, Matthew S; Widjaja, Christella E; Kanbar, Jad N; Olvera, Jocelyn G; Tysl, Tiffani; Quezada, Lauren K; Boland, Brigid S; Huang, Wendy J; Murre, Cornelis; Goldrath, Ananda W; Yeo, Gene W; Chang, John T.
Afiliação
  • Kurd NS; Department of Medicine, University of California San Diego, La Jolla, CA, USA.
  • He Z; Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA, USA.
  • Louis TL; Division of Biologic Sciences, University of California San Diego, La Jolla, CA, USA.
  • Milner JJ; Department of Medicine, University of California San Diego, La Jolla, CA, USA.
  • Omilusik KD; Division of Biologic Sciences, University of California San Diego, La Jolla, CA, USA.
  • Jin W; Division of Biologic Sciences, University of California San Diego, La Jolla, CA, USA.
  • Tsai MS; Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA, USA.
  • Widjaja CE; Department of Medicine, University of California San Diego, La Jolla, CA, USA.
  • Kanbar JN; Department of Medicine, University of California San Diego, La Jolla, CA, USA.
  • Olvera JG; Department of Medicine, University of California San Diego, La Jolla, CA, USA.
  • Tysl T; Department of Medicine, University of California San Diego, La Jolla, CA, USA.
  • Quezada LK; Department of Medicine, University of California San Diego, La Jolla, CA, USA.
  • Boland BS; Department of Medicine, University of California San Diego, La Jolla, CA, USA.
  • Huang WJ; Department of Medicine, University of California San Diego, La Jolla, CA, USA.
  • Murre C; Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA, USA.
  • Goldrath AW; Division of Biologic Sciences, University of California San Diego, La Jolla, CA, USA.
  • Yeo GW; Division of Biologic Sciences, University of California San Diego, La Jolla, CA, USA.
  • Chang JT; Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA, USA. geneyeo@ucsd.edu changj@ucsd.edu.
Sci Immunol ; 5(47)2020 05 15.
Article em En | MEDLINE | ID: mdl-32414833
ABSTRACT
During an immune response to microbial infection, CD8+ T cells give rise to distinct classes of cellular progeny that coordinately mediate clearance of the pathogen and provide long-lasting protection against reinfection, including a subset of noncirculating tissue-resident memory (TRM) cells that mediate potent protection within nonlymphoid tissues. Here, we used single-cell RNA sequencing to examine the gene expression patterns of individual CD8+ T cells in the spleen and small intestine intraepithelial lymphocyte (siIEL) compartment throughout the course of their differentiation in response to viral infection. These analyses revealed previously unknown transcriptional heterogeneity within the siIEL CD8+ T cell population at several stages of differentiation, representing functionally distinct TRM cell subsets and a subset of TRM cell precursors within the tissue early in infection. Together, these findings may inform strategies to optimize CD8+ T cell responses to protect against microbial infection and cancer.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Análise de Sequência de RNA / Linfócitos T CD8-Positivos / Análise de Célula Única Limite: Animals / Female / Humans / Male Idioma: En Revista: Sci Immunol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
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PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Análise de Sequência de RNA / Linfócitos T CD8-Positivos / Análise de Célula Única Limite: Animals / Female / Humans / Male Idioma: En Revista: Sci Immunol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos