Advantages of formate dehydrogenase reaction for efficient NAD<sup>+</sup> quantification in biological samples.

Artiukhov, Artem V; Pometun, Anastasia A; Zubanova, Sofia A; Tishkov, Vladimir I; Bunik, Victoria I

Advantages of formate dehydrogenase reaction for efficient NAD⁺ quantification in biological samples.

Artiukhov, Artem V; Pometun, Anastasia A; Zubanova, Sofia A; Tishkov, Vladimir I; Bunik, Victoria I.

Afiliação

Artiukhov AV; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 119234, Moscow, Russia; A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119234, Moscow, Russia.
Pometun AA; Department of Chemistry of Lomonosov Moscow State University, 119234, Moscow, Russia; Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 119071, Moscow, Russia.
Zubanova SA; Department of Chemistry of Lomonosov Moscow State University, 119234, Moscow, Russia.
Tishkov VI; Department of Chemistry of Lomonosov Moscow State University, 119234, Moscow, Russia; Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 119071, Moscow, Russia. Electronic address: vitishkov@gmail.com.
Bunik VI; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 119234, Moscow, Russia; A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119234, Moscow, Russia; Department of Biochemistry, Sechenov University, 119991, Moscow, Russia. Electro

Anal Biochem ; 603: 113797, 2020 08 15.

Article em En | MEDLINE | ID: mdl-32562604

Assuntos

Fluorometria/métodos; Formiato Desidrogenases/química; Formiato Desidrogenases/metabolismo; NAD/análise; Animais; Química Encefálica; Mitocôndrias/química; NAD/química; NAD/metabolismo; Ratos; Ratos Wistar; Sensibilidade e Especificidade; Extratos de Tecidos/análise

Palavras-chave

Cerebral cortex; Enzymatic assay; Formate dehydrogenase; Methanol-acetic acid extract; NAD(+)

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fluorometria / Formiato Desidrogenases / NAD Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Anal Biochem Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Federação Russa

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