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Combi-CRISPR: combination of NHEJ and HDR provides efficient and precise plasmid-based knock-ins in mice and rats.
Yoshimi, Kazuto; Oka, Yuichiro; Miyasaka, Yoshiki; Kotani, Yuko; Yasumura, Misato; Uno, Yoshihiro; Hattori, Kosuke; Tanigawa, Arisa; Sato, Makoto; Oya, Manami; Nakamura, Kazuhiro; Matsushita, Natsuki; Kobayashi, Kazuto; Mashimo, Tomoji.
Afiliação
  • Yoshimi K; Division of Animal Genetics, Laboratory Animal Research Center, Institute of Medical Science, The University of Tokyo, Tokyo, 108-8639, Japan.
  • Oka Y; Division of Genome Engineering, Center for Experimental Medicine and Systems Biology, Institute of Medical Science, The University of Tokyo, Tokyo, 108-8639, Japan.
  • Miyasaka Y; Department of Anatomy and Neurosciences, Graduate School of Medicine, Osaka University, Osaka, 565-0871, Japan.
  • Kotani Y; Department of Child Development, United Graduate School of Child Development, Osaka University, Osaka, 565-0871, Japan.
  • Yasumura M; Institute of Experimental Animal Sciences, Graduate School of Medicine, Osaka University, Osaka, 565-0871, Japan.
  • Uno Y; Institute of Experimental Animal Sciences, Graduate School of Medicine, Osaka University, Osaka, 565-0871, Japan.
  • Hattori K; Department of Anatomy and Neurosciences, Graduate School of Medicine, Osaka University, Osaka, 565-0871, Japan.
  • Tanigawa A; Institute of Experimental Animal Sciences, Graduate School of Medicine, Osaka University, Osaka, 565-0871, Japan.
  • Sato M; Institute of Experimental Animal Sciences, Graduate School of Medicine, Osaka University, Osaka, 565-0871, Japan.
  • Oya M; Institute of Experimental Animal Sciences, Graduate School of Medicine, Osaka University, Osaka, 565-0871, Japan.
  • Nakamura K; Department of Anatomy and Neurosciences, Graduate School of Medicine, Osaka University, Osaka, 565-0871, Japan.
  • Matsushita N; Department of Child Development, United Graduate School of Child Development, Osaka University, Osaka, 565-0871, Japan.
  • Kobayashi K; Department of Integrative Physiology, Nagoya University Graduate School of Medicine, Nagoya, 466-8550, Japan.
  • Mashimo T; Department of Integrative Physiology, Nagoya University Graduate School of Medicine, Nagoya, 466-8550, Japan.
Hum Genet ; 140(2): 277-287, 2021 Feb.
Article em En | MEDLINE | ID: mdl-32617796

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plasmídeos / Técnicas de Introdução de Genes / Reparo do DNA por Junção de Extremidades / Reparo de DNA por Recombinação / Sistemas CRISPR-Cas Limite: Animals Idioma: En Revista: Hum Genet Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Japão

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plasmídeos / Técnicas de Introdução de Genes / Reparo do DNA por Junção de Extremidades / Reparo de DNA por Recombinação / Sistemas CRISPR-Cas Limite: Animals Idioma: En Revista: Hum Genet Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Japão