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Molecular characterization and pathogenicity of highly pathogenic fowl adenovirus serotype 4 isolated from laying flock with hydropericardium-hepatitis syndrome.
Yin, Dongdong; Xue, Mei; Yang, Kankan; Xiong, Xiuqi; Geng, Shu; Tu, Jian; Song, Xiangjun; Shao, Ying; Wang, Guijun; Qi, Kezong.
Afiliação
  • Yin D; College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, PR China; Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Hefei, 230036, PR China.
  • Xue M; College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, PR China; Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Hefei, 230036, PR China.
  • Yang K; College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, PR China; Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Hefei, 230036, PR China.
  • Xiong X; College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, PR China; Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Hefei, 230036, PR China.
  • Geng S; College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, PR China; Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Hefei, 230036, PR China.
  • Tu J; College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, PR China; Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Hefei, 230036, PR China.
  • Song X; College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, PR China; Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Hefei, 230036, PR China.
  • Shao Y; College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, PR China; Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Hefei, 230036, PR China.
  • Wang G; College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, PR China; Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Hefei, 230036, PR China.
  • Qi K; College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, PR China; Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Hefei, 230036, PR China. Electronic address: qkz@ahau.edu.cn.
Microb Pathog ; 147: 104381, 2020 Oct.
Article em En | MEDLINE | ID: mdl-32739401
ABSTRACT
Hydropericardium-hepatitis syndrome (HHS) is an important emerging disease responsible for huge economic losses to the poultry industry in China. HHS primarily affects 20 to 60-day-old broilers and rarely occurs in laying flock. In this study, the highly pathogenic fowl adenovirus (FAdV) strain, AH-F19, was isolated from the liver samples of 120-day-old laying flock with HHS and its phylogenetic information, genetic mutations, and pathogenicity was evaluated. The phylogenetic analysis revealed that AH-F19 belonged to the FAdV serotype 4 (FAdV-4) cluster, however, 100K differs from the other FAdV-4 strains and is divided into different branches. Amino acid variations in fiber-2 for pathogenic isolates and non-pathogenic isolates indicated that D219, T300, and T380 may not be responsible for virulence. Animal experiments revealed AH-F19 to be a highly pathogenic isolate that can cause 100% mortality in three-week-old specific pathogen-free (SPF) chickens, which exhibited typical hydropericardium and hepatitis. Microscopically, the presence of basophilic intranuclear inclusion bodies in hepatocytes, fractured heart muscle fibers, as well as kidney degeneration and necrosis was observed. Collectively, these findings enriched our understanding of FAdV-4 pathogenicity and provided a reference for further exploration into its pathogenicity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Aves Domésticas / Infecções por Adenoviridae / Hepatite Limite: Animals País/Região como assunto: Asia Idioma: En Revista: Microb Pathog Assunto da revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Aves Domésticas / Infecções por Adenoviridae / Hepatite Limite: Animals País/Região como assunto: Asia Idioma: En Revista: Microb Pathog Assunto da revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Ano de publicação: 2020 Tipo de documento: Article