A clinically validated human capillary blood transcriptome test for global systems biology studies.
Biotechniques
; 69(4): 289-301, 2020 10.
Article
em En
| MEDLINE
| ID: mdl-32772558
To prevent and treat chronic diseases, including cancer, a global application of systems biology is needed. We report here a whole blood transcriptome test that needs only 50 µl of capillary (fingerprick) blood. This test is suitable for global applications because the samples are preserved at ambient temperature for up to 4 weeks and the RNA preservative inactivates all pathogens, enabling safe transportation. Both the laboratory and bioinformatic steps are automated and performed in a clinical lab, which minimizes batch effects and creates unbiased datasets. Given its clinical testing performance and accessibility to traditionally underrepresented and diverse populations, this test offers a unique ability to reveal molecular mechanisms of disease and enable longitudinal, population-scale studies.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Capilares
/
Biologia de Sistemas
/
Imagem Corporal Total
/
Transcriptoma
Limite:
Humans
Idioma:
En
Revista:
Biotechniques
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Estados Unidos